Abstract: The effect of Virginia tobacco specialized fertilizer (N.14.76%、P2O5.7.33%、K2O.18.66%) amount and application methods on seedling quality were evaluated in floating tray by the aid of seedling emergence and survival rates, main agronomic traits, dry matter and nutrient element contents. The results showed that N, P, K and Ca contents were the highest in root, higher in stem, low in leaf when 0.9kg fertilizer per standard pool (34 trays: 66 x 34 x 5cm in dimension) was applied for two times: 60% in seedling emergence period and 40% in big~cross period, indicating the best quality of tobacco seedling according to stem height and diameter, dry root and stem weights and the biggest ratio of root to overground parts.