Abstract: In this paper, a specially made root chamber system with two compartments and nine gas collection pipes was used to collect the N2O in soil air of nine different spatial position in the root chamber. The following treatments were also used: (1) Two selective nitrification inhibitors, C2H2 and DCD in low concentration were used to study the nitrification process under different NH4+-N levels and its contribution to N2O release. (2) High concentration C2H2, which inhibitates the reduction from N2O to N2 in the denitrification process, was used to determine the denitrification process under different NO3--N levels and its contribution to N2O release. The root chamber system combined with the use of inhibitors was an effective method to study nitrogen transform processes in soil, N2O release process ang the effect of plants on it.