Abstract: Spilt-plot design was employed to study the effect of the co-application of organic fertilizer and nitrogen fertilizer on the yield and economic benefit of greenhouse cucumber,and also the nutrient quality and nitrate content of it. The result showed that the yield of cucumber was increased by increasing the rate of organic fertilizer and nitrogen fertilizer application,and the ones were M2N3、M3N1、M3N2 which had a higher economic benefit and investment income rate.The nitrite content of cucumber was remarkly increased by increasing the amount of nitrogen application,but the content of Vc and deoxidized sugar was reduced when high amount of nitrogen fertilizer(N3) was applied. With the co-application of organic fertilizer and nitrogen fertilizer,the nitrite content was decreased and the synthesize of Vc and deoxidized sugar was promoted in greenhouse cucumbers.The combination of the organic fertilizer application 45t/hm2(M2)or 90t/hm2(M3) and the nitrogen fertilizer application 450kg/hm2(N1) or 750kg/hm2(N2) was the better one.Considering the quality of cucumber and the economic and ecological benefits,the best combination was the deal which had a moderate amount 90t/hm2 in organic fertilizer application and a low amount 450kg/hm2 in nitrogen fertilizer application.