Establishment of Regeneration System from Leaves of 3 Populus Varieties

Abstract

Abstract: This paper deals with regeneration system from leaves of 3 Populus varieties (Populus deltoides cv.’zhonglin-2001’, Populus deltoids Bartr. cv. Lux × P . euramericana（Dode）Guineir cv. I-45 / 51, Populus deltoides cl. Nankang) with fifteen induced callus media, six differentiation media and seven rooting media. The effects of genotype, hormone concentration and explants condition were investigated. The results indicated that the effect on induced callus and differentiation of the three Populus varieties were related to the ratio of NAA to 6-BA. The ratio of NAA to 6-BA on induced callus was 4:1~5:1. The stoma number of leaves face and back, induced callus and plant regeneration of different varieties were different. There were significant effects on induced callus from leaves which were culture on medium with leaves face up or back up, leaves edge or middle.

CLC Number:

S722.3

Shen Zhougao, Xiang Yan, Cai Cheng, Wu Daqiang. Establishment of Regeneration System from Leaves of 3 Populus Varieties[J]. Chinese Agricultural Science Bulletin, 2006, 22(11): 90-90.

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