Abstract: According to swine vesicular disease virus (SVDV) gene published and RT-PCR assay for the diagnosis and characterization of SVDV reported, 2 sets of primers to detect SVDV were designed in the conserved VP1 gene of SVDV which is conserved and specific. The production of the RT-PCR assay is 251bp and 366bp, subsequently cloned into pMD18-T Vector. The nucleotide of production was compared with the corresponding sequences of published sequence of SVDV. The nucleotide homology was 100%. Suggest that it is gene of SVDV. Specificity was tested by that amplification of FMDV、VSV、BHK21 cell was negative. The dilution of 10-4 SVDV RNA can be detected. Suggest that it has high sensitivity. The RT-PCR method is rapid, sensitive and specific for detecting SVDV.