Abstract: Some physiological characteristics and regeneration ability of Agrostis stolonifera Penncross and Yuexuan No.1under high temperature(38℃) were studied. The content of chlorophyll and ascorbic acid dropped under the high temperature stress, and the chlorophyll content in leaves of Yuexuan No.1 dropped slower than Penncross, but the content of ascorbic acid in leaves of Yuexuan No.1 was significantly higher than Penncross; The content of MDA and proline increased, and the leaf MDA of Yuexuan No.1 was significantly lower than Penncross, but the proline content in leaves of Yuexuan No.1 was significantly higher than Penncross. The activity of antioxidant enzymes(POD, SOD ＆ CAT) in leaves of Yuexuan No.1 was significantly higher than Penncross under the high temperature stress. The creeping stem regeneration ability of Yuexuan No.1 was significantly higher than Penncross on the first six days of high temperature stress.