Abstract: Several peach varieties were used as materials. The leaves were disposed in low temperature and the relative electrolyte leakage has been determined. The flowers were disposed in different low temperature gradient and then were observed whether the pistil turned brown. The results showed that the varieties with low relative electrolyte leakage of leaves appeared strong cold resistance. The descending order of cold resistance of different varieties was:‘Yunong2’>‘Hongxue’and‘Qiumi’>‘Yuhua’.This was different from the results those have been mensurated with dormiens branches. It suggested that it should be discreet when relative electrolyte leakage of branches was used to identify cold resistance of Peach.