Abstract: In order to study the clonal architecture of Lotus (Nelumbo nucifera Gaertn), the leaf size, petiole height, internode length, branching angle, branching node position, enlarged internode position, etc., were investigated with three or more cultivars growing under different field conditions, including paddy fields and fish ponds. The results showed that the branching pattern of lotus rhizome was monopodium and the branches were horizontally alternate with branching angle from 62.6°to 98.7, and was varying from different cultivars. The petiole height and diameter, the internode length and diameter of rhizome all increased gradually with the number of internodes increased, and that is a result different from other reports. The enlarged internode position on main rhizome can be used as one of the indicators of maturity. This paper also showed that there are obviously differences of clonal architectures among different cultivars and different field conditions.