Abstract: The impact of high temperature and abamectin to Tetranychus cinnabarinus (Boiduval) and the expression of heat shock protein were investigated in laboratory. The results showed that T. cinnabarinus was reared at 34℃ for a year, named as heat resistant strain (HR), its LC50 to abamectin increased to 2.1396 folds compared with the susceptible strain (Ss). T. cinnabarinus was selected with abamectin for 24 generation, named abamectin resistant strain (AR), when it was exposed to 48℃ for 1h, the mortality was approximately same as Ss, but the mortality of HR is significantly lower. Heat shock protein was detected by SDS-PAGE, the results showed that compared with the Ss, the expression of 28.5 and 26.8 kDa protein was increased. When AR was exposed to 43℃, the 98.6kDa expression was significant increased, while the 54.9kDa expression was the least. When T. cinnabarinus was exposed to 43℃, 75.9kDa expression in all strains was increased, especially for HR. The change of protein expression might be relative to resistance to high temperatures and abamectin.