Abstract: In order to increase germination ratio of the seeds, enhance stress-resistance of seedling, accelerate popularization of variety, improve quality of turf, this experiment adopted chitosan of different concentrations to deal with the Dichondra repens Forst. seeds and carried out the sprouting and planting test. The test investigated the germination ratio of seed, plant height, fresh weight of shoot, fresh weight of root, root length and ratio of root to shoot. At the same time, the activities of PAL, SOD, POD and PPO of seedling from different stages (7d, 14d, 21d) were measured. The results showed that the chitosan of other concentrations promoted evidently the seed germination and seedling growth with the exception of 0.8 g/L in germination, and with the increasing of concentration, the activities of four enzymes all increased. The concentration of 0.5g / L was the best. With the furthering concentration, the inhibitive effect appeared. The experiment indicated that the seeds were soaked in chitosan of feasible concentration, the germination ratio of the seeds, the growing state and the stress-resistance of the seedlings could be improved and the turf establishment time could be shorted.