Study on Restrain Inhibition of Flumorph to Phytophthora Sojae

Abstract

Abstract: One of the main measures to combat Phytophthora sojae was using Metalaxyl. Because of its role in a single site and easy mutant bacteria resistant, it is necessary to select a new fungicide metalaxyl alternative Phytophthora root rot. Using flumorph, the bioactivity of 3 fungicides against different stages of Phytophthora sojae Ps411 was compared in vitro. The results showed that flumorph had significant inhibitory effect on the 4 different growth stages of the strain, with 50% effective concentration (EC50) values was 0.1906μg/mL. Their 95% effective concentration (EC95) value was 0.4735 μg/mL. Flumorph at the concentration of 0.5 μg/mL, can lead the inhibit rate of growth of mycelium, formulation of sporangium and germination of zoospore of Phytophthora sojae to 100%, the concentration was lower than Apron XL, Metalaxy which lead to the same inhibition rate. It indicated that Flumorph can obviously inhibit the growth of P.sojae in vitro, and effect is better than that of Apron XL and Metalaxy.

CLC Number:

S481+.9

References

[1] 刘武成,李志念,张越,等.氟吗啉药效应用研究[I]——室内生物活性测定.农药,1999,38(4):10-13. [2] 朱书生,刘西莉.新型杀菌剂氟吗啉的作用机制初探.中国植物病理学学会2005年年会论文集. [3] 孙利,刘永春.50%氟吗啉+乙膦铝防治温室黄瓜霜霉病药效试验.农药,2005,44(4):184-185. [4] 李金先,王国荣,楼曼庆,等.60%氟吗?锰锌防治黄瓜霜霉病田间药效试验.农药,2003,42(3):28 [5] 黄立华,刘君丽,司乃国,等.辣椒疫病菌对氟吗啉的抗药性研究.农药,2004,43(6): 261-262. [6] 刘武成,李志念,张宗俭,等.氟吗啉及其混剂的杀菌活性及开发前景.浙江化工,2000,31(增刊):87-89. [7] 沈崇尧,苏彦纯.中国大豆疫霉根腐病的发现及初步研究.植物病理学报,1991,21(4):298 [8] 左豫虎,薛春生,刘惕若.杀菌剂对大豆疫霉菌生长的抑制作用.黑龙江大学自然科学学报,2002,19(1):106-108. [9] Bhat R G, Mcblain B A, Schmitthenner A F. The inheritance of resistance to metalaxyl and to flurophenylalaine in matings of homothallic Phytophthora sojae. MycolRes,1993,97(7):865-870 [10] 王源超,郑小波,陆家云.疫霉菌对甲霜灵抗性遗传研究.上海农学院学报,1995,13(增):1-5. [11] Joseph M C and Coffey M D. Development of laboratory resistance to metalaxyl in Phytophthora citricola. Phytopathol, 1984, 74 (2):1411-1414 [12] Serrhihi N , Maraite H, Meyer J A. In vitro selection of Phytophthora citrophthora strains resistant to metalaxyl. Eppo Bulletin, 1985, 15 (4):443- 449. [13] 左豫虎,侯巨梅,康振生,等.大豆疫霉菌抗甲霜灵特性研究.菌物学报,2005, 24(3):422-428. [14] 臧忠婧,左豫虎,刘惕若,等.大豆疫霉菌的分离,鉴定及菌株致病力的测定.黑龙江八一农垦大学学报,2000,12(1):37-42. [15] 郑小波.疫霉菌及其研究技术.中国农业出版社,北京:1997,3. [16] 左豫虎,薛春生,刘惕若.杀菌剂对大豆疫霉菌生长的抑制作用.黑龙江大学自然科学学报,2002,19(1):106-108. [17] 左豫虎,减忠蜻,刘惕若.影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件.植物病理学报,2001,31(3):241-245. [18] 严占勇,肖崇刚,易龙,等.防治辣椒疫病的芽孢菌株的筛选及其抑菌效果的测定.中国蔬菜,2005(6):18-20. [19] 尹敬芳,曹锦,李健强,等. 杀菌剂对辣椒疫霉不同形态茵体的毒力差异. 农药学学报,2005,7(3):227-232.