Abstract: Coenzyme Q10 is a very good biochemicals, which has important physiological function. And it is also natural oxidation and cell metabolism activating agent of activated cellular breathing, which is produced by cell oneself. The author has studied the technics that the extraction and isolation of Coenzyme Q10 from cattle heart. Coenzyme Q10 is obtained through saponification, extraction with petroleum benzine, chromatography with silica gel and crystallization with ethanol. And under the optimal conditions, concentration of CoQ10 that determinated with standard curve chart was 91.2%.