Abstract: 【OBJECTIVE】The main object of this article was to develop and creat new antiviral pesticide for controlling tobacco virus diseases, consequently it could provide guidance to control tobacco mosaic virus diseases during tobacco production process【METHOD】The experiment treatments were Brassinolide 400×, 8% Ninnanmycin AS 800× and CK, respectively, each treatment replicated 3 times, the design of random block【RESULTS】The field experiments on controlling tobacco and cucumber mosaic virus diseases were fulfilled to validate the control efficacy of the plant growth regulator (Brassinolide) and Ninnanmycin, in Qian-jiang tobacco growing area, Chongqing. The result showed that both of the pesticides had inhibitory effect to tobacco mosaic virus diseases and had the effect of accelerating tobacco growth, of which the mean control efficacy were both about 70%【CONCLUSION】Brassinolide not only had the ability of the plant growth regulator to accelerate tobacco growth, but also was an ideal pesticide to control tobacco mosaic virus .