Abstract: [Objective]To approach the toxin mechanism of cadmium on the deveolpment of hens ovaries, and to explain the morbidity mechanisms of cadmium poisoning in the molecular level, in this way to improve the environmental protection consciousness of human, so we can avoid the cadmium poisoning. [Method]we separately added 140mg/kg CdCl2、210mg/kg CdCl2 in the ration to establish the model of the sub-chronic cadmium poisoning. After 20d、40d and 60d, hens ovaries were taken out and weighed to detect the activity of Na+-K+-ATPase, Mg2+-ATPase and Ca2+-ATPasesplenic. [Results]The results show that the activities of Na+-K+-ATPase, Mg2+-ATPase and Ca2+-ATPase in the control group were increasing with the development of its age ,and there were no significant differences among each time point. The enzyme activities above in the cadmium group significantly decreased with the time increasing comparing with the control group, and there is a time-dose relation. 【CONCLUSION】The results also show cadmium can depress the developing of ovarian and decrease the activity of ATPase, and furtherly the relativities has been observed between them.