Abstract: 【OBJECTIVE】In order to learn the effects of carbon dioxide on the growth and Cu accumulation of Indian Mustard growing on copper contaminated Soil. 【METHOD】Indian mustard grew on soil containing different Cu concentrations in greenhouse were conducted in the present thesis. Two levels of CO2 treatments were used in homemade close CO2 chambers. 【RESULTS】After short-term and large numbers of experiments, it was found that enriching CO2 promoted growth and development of plants. Without the addition of Cu, the Indian mustard at the CO2 concentration of 1200μL/L and 800μL/L came into blossom ahead of the control for 3d and 2d, respectively. The heights of plants with and without CO2 enrichment were different markedly. The maximum areas, the numbers and the dry weights of the plant leaves with CO2 enrichment were increased than the control. The leaf numbers of plants under CO2 concentration of 1200μL/L had very significant difference with the control. The leaf areas and the root weights had significant difference and the dry weights of shoots increased prominently. With CO2 enrichment, significant increases in Cu accumulation were occurred in Indian mustard and Cu concentrations of the roots were higher than the control. Furthermore, there was very significant difference between Cu concentrations at two CO2 concentration levels, but it is not a linear relation between the concentrations of CO2 fertilization and the accumulation of Cu in the plant. Under CO2 concentration of 800μL/L and Cu concentration of 100mg/kg, the mean concentration in stems and leaves of Cu reached the maximum of 13696mgCu/Kg dry weight in stems and 4589mg Cu/kg dry weight in leaves, respectively. Meanwhile, the copper leaf/root ratio significantly increased with CO2 fertilization. The results showed that carbon dioxide fertilization was propitious to the transportation of Cu from roots to stems. 【CONCLUSION】CO2 fertilization can promote the growth of Indian mustard and increase the accumulations of Cu in the plants. Meanwhile, the copper leaf/root ratio significantly increased with CO2 fertilization.