Abstract: It was obtained aseptic line derived from seed condition of Sapashnikovia divaricata (Turcz.) Gsch ischk. It studied affected callus shaped by different explant, different basic medium, different proportion of incretion. Different proportion of incretion and bring up number of days effected proliferated callus. Results showed that be sieving the basic medium; the best basic medium was MS substrate when callus by inducement; the root’s inducement percent was highest, it was 99.3%, but it wrought callus quality of a material wasn’t good; the leaf’s inducement percent was only lower than the root’s inducement percent, it was 84.6%, and quality of a material was fall apart, showed pea green, it is fittest to studied. It could appear callus only 7d in MS+2,4-D0.6+6-BA0.5 substrate. Orthogonal plan experimentation Results showed: The 6-BA and NAA had obvious accelerate effect when its callus was proliferated, the proliferated speed was soonest when kept up’s number of days was 30d.