Abstract: To develop a nove1 sensitive, specific and lower cost method applicable for detection of VTEC. Two primers were designed respectively based on the gene of VT1 and VT2,and the VTEC gene were detected by PCR which includes SYBR Green I, a double-stranded DNA-specific fluorescent dye, and the sequence specific primers, The results showed that the method possessed high specificity and sensibility for detection of VTEC and achieved 10cfu/g.The method could detect VTEC from clinical specimen and food. The method was quickly，convenient, accurate, high efficient and in favor of early diagnosis and detection of VTEC.