Abstract: A simple method was developed for the detection of Hog Cholera Virus (HCV) in clinical samples using colloidal gold immunochromatographic assay, and we can discriminate violent and weak virus by using this method. Protein E2 of Hog Cholera Virus that was expressed in E.coli acted as attenuated HCV antigen and the violent HCV that was cultivated and purified by using PK15 cells acted as violent HCV antigen. By using these materials, we developed the immunochromatographic assay test paper for detecting HCV. The test paper still had good stability after it was destroyed by the temperature of 37℃, the sensitivity of the test paper was better in certain degree than the American IDEXX enzyme linked immunity reagent and the indirect agglutination through comparison; experimented with several kinds of masculine blood serums of virus and disease germs, and it demonstrated that the test paper had better specificity. The development of this test paper provides a more practical method for the fast diagnosis of HCV.