Abstract: “3S” have become the important tools in soil resource investigate and management. The author states the application of “3S” techniques in soil spot arrangement and sampling respectively taking the soil fertility monitoring sampling as the example. The application includes updating the soil spot arrangement base map using RS and GIS techniques; primary arranging and adjusting the soil spot with gridding method by the aid of GIS technique, and then exporting the spot location sketch map and spot coordinates; using the GPS for spot position or correcting the spot arrangement map and coordinates according to the spot factual condition in outdoor soil sampling. The results shows: (1) Comparing the soil map in 1982 and updated soil map, the data indicated that the obvious have change happened of soil amount and soil type with the development of urbanization. (2) Using soft MapGIS6.5 created 2.5×2.5 km grid and primary arranged 110 soil spot. (3) Selecting 105 soil sample with the guide of GPS for spot position, and obtaining the actual geography information of soil spot. The application of “3S” in soil spot arrangement and sampling will be the base of soil research in future, i.e.，farther soil property analysis from spot scale to area scale.