Abstract: In order to explore the effect to fertilizer use efficiency , optimum way and yield increasement mechanism of plasma treatment on soybean seed, fertilizer use efficiency experiment of plasma treatment on soybean seed was done. There were five dosages, seven treatments and three duplicates in the experiment, the treatments were arranged randomly. According to the investigation during the seedling stage, the number of root were treated are more 1.8-2.9 than CK1, the plant height was not significant among all the treatments. The results showed that plasma treatment at the dosage of 1.0A×2 and 1.5A×2 could enhance nutrient uptake of soybean by chemical analyzed and yield surveying on dry matters during pod stage and maturity stage, the total fertilizer of N+P2O5+K2O was enhanced more than 10.0% compared to CK1,The use efficiency of N、P、K was enhanced by 25.2%~32.9%,3.0%~6.7% and 7.5~13.5%.It was clarified that plasma treatments at the dosage of 1.0A×2 could increase yield significantly by 11.0% than CK1.