Abstract: The planting of Crocus sativus in Guizhou province is short, and the studies on occurrence and damage of diseases are relatively scarce. At present, the stem rot disease of Crocus sativus has become a serious disease for its damage. Through outdoor investigation and separation, training, identification of pathogens from disease tissue, the paper presents the experimental results: the stem rot disease caused by interactive infection of Fusarium sp. and Anthracnose sp. .