Antioxidative System Characteristics of Sweet Potato Transferred both Cu/Zn Superoxide Dismutase and Ascorbate Peroxidase Gene under Water Stress and Rewatering

Abstract

Abstract: Two strains, Cu/Zn SOD and APX gene transferred sweet potato (TS) and non-transgenic sweet potato（Ipomoea batatas L.）（NT），were used as experimental materials to study the effects of Water stress and rewatering on antioxidative system characteristics of sweet potatoes. The result showed that the activities of SOD of both TS and NT first increased and then decreased under water stress, but the activity of SOD of TS was always higher than that of NT, under 24h water stress, the activity of SOD of TS was 20% higher than that of NT. The activities of SOD of TS and NT both decreased after rewatering. Under water stress, the activity of APX of TS firstly increased and then decreased; in NT it firstly decreased and then increased. After rewatering, the activity of APX of both TS and NT firstly increased and then decreased, after12h rewatering, the activity of APX of TS was 50% higher than it of NT (Fig.1). When PEG treatments were imposed for 24h and 36h in TS, the AsA content increased by 12% and 53%, however, the AsA content was in NT increased firstly and then decreased. After PEG treatments for 0h, 12h and 24h, AsA content in TS was higher by 3 %, 18 % and 37 %, than that in NT (p<0.05), respectively (figure 2). Recovery by no PEG solution, AsA content decreased in both TS and NT. Recover for 12h, 24h, AsA content in TS was 45% and 37% higher than that in NT (p<0.05). Under the no stress and PEG treatments for 12h and 24h, CAR content in NT was higher by15 %,21% and 13 %, than that in TS (p<0.05), respectively. After PEG treatments for 36h, CAR content in TS and NT was almost the same. Recovery in on PEG solution, CAR contents decreased in TS and NT. Recovered for 12h, CAR content in NT was 11% higher than in TS Under water stress, the membrane structural stability of TS was better than it of NT, after 24h water stress and 12h rewatering, the MDA content of NT were 20% more than it of TS (Fig.5). The result showed that Transferred both Cu/Zn SOD and APX gene，the antioxidative system of the transgenic sweet potato had a higher ability to protect and recover the antioxidative system of the plant.

CLC Number:

Q945.17

Li JianMei, Deng XiPing. Antioxidative System Characteristics of Sweet Potato Transferred both Cu/Zn Superoxide Dismutase and Ascorbate Peroxidase Gene under Water Stress and Rewatering[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 616-616.

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