Abstract: In this present test, a range of hybrid shepherd dogs were selected firstly. Afterwards, the effects of FCS，PBMC，PHA-P of different concentration on promote lymphocytes proliferation were tested by MTT test. The result of experiment demonstrate that the best levels combination of canine lymphocyte，calf blood serum were 5×106 unit/ml， 10% respectively. In the lymphocytic transfer test, PHA-P at dosage of 12.5μg/ ml performed facilitation effects for canine lymphocytic proliferation (p>0.05). ConA at 0.5~2.5μg/ mL markedly proliferated T lymphocytes (p<0.05), with the best effect at 2.5μg/ ml.