Abstract: In this paper, the branches of Chinese jujube and its plantlets were used as test materials to establish the optimum system of isolating total RNA. Because Chinese jujube are rich in polysaccharides and polyphenol compounds, the CTAB method were improved by added polyvinylpyrrolidone(PVP) and β-ME to remove polyphenol compounds, added KAc and ethanol to remove polysaccharides and added LiCl to deposit RNA. The bands of the isolated RNA were regular and clear. The A260/A280 of total RNA were between 1.8~2.0 and A260/A230 were more than 2.0. That proved the method could extract high-purity and highly-complete RNA at a high productivity and could remove protein, polysaccharides and polyphenol compounds completely. The result showed that the improved CTAB-LiCl method is suitable to isolate total RNA of Chinese jujube.