The growth of Gerbera plantlets on the sucrose-free media and effect of different closures on it in vitro

Abstract

Abstract: The effects of sucrose and sucrose -free media, different closures on the growth , fresh weight increasment , dry matter and rooting of Gerbera jamesonii plantlets in vitro were studied. Plantlets of rooting-stage could survive on the sucrose-free media .which showed the characteristic of photoautotrophical growth , plantlets grown in the vessel closed with the gas-permeable film closure have greater survival rate , fresh weight ,dry matter and rooting rate than those closed with airtight film. gas-permeable film closure also increase the proliferation rate of explants. The result showed the potential of controlling culture enviroment to improve the efficiency of micropropagation and quality of plantlets through photoautotrophical growth.

Liao feixiong, Li ling, Yao cuixian, Gou zhongxiao. The growth of Gerbera plantlets on the sucrose-free media and effect of different closures on it in vitro[J]. Chinese Agricultural Science Bulletin, 2004, 20(4): 211-211.

References

1. 廖飞雄，王碧青、吕复兵等园艺植物组织培养中光自养生长研究及应用，见：中国园艺学会第九届学术年会论文集，北京，中国科学技术出版社，2001，398-401，
2. Kozai, T., Kubota, C ,Jeong B R Enviromental control for the large-scale production of plants through in vitro techniques Plant Cell Tissue and Organ Culture 1997,51(1):49-56
3. Jeong, B. R. Fujiwara, K. and Kozai, T. Environmental Control and Photoautotrophic Micropropagation Horticultural Review 1995,vol 17 : 125-172
4. 肖玉兰，张立力，张光怡等，非洲菊无糖组织培养技术的应用研究，园艺学报，1998，25（4）：408-410
5. Lentini，Z., Mussell，H.； Mutschler， M.A. et. al Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L. Plant Sci.1988,54:75-81
6. Monette,P. Micropropagation of Kiwifruit using non-axenic shoot tips，Plant Cell Tissue Organ Cult.，1986.6:73-82
7. Mackay ,W.A. and Kitto，S.L. Factors affecting in vitro shoot proliferation of French tarragon， J. Am. Soc. Hort, 1988,113:282-287
8. McClelland, M. T. and Smith，M.A.L., Vessel types, closure, and explant orientation influence in vitro performance of five woody species. HortScience, 1990，25:797-800
9. Blazkova, A., Ullmann, Z., and Josefusova, Z., et al The influence of gaseous phase on the growth of plants in vitro :the effect of different types of stoppers , Acta Hort.1989,251 :209-214
10. Dillen, W. and Buysens，S., A simple technique to overcome vitrification in Gypsophila paniculata L. Plant Cell Tissue Organ Cult., 1989,19:181-188
11. Hakkaart, F.A and Versluijs，J.A., Some factors affecting glassiness in carnation meristem tip cultures , Neth. J. Plant Pathol.1983,89:47-53
12. Kozai,T. and Sekimoto，K., Effect of the number of air changes per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantlets in vitro Environ. Control Biol.1988,16:21-29
13. Tsuro, M.; Koda, M.; Inoue, M. Efficient plant regeneration from multiple shoots formed in the leaf-derived callus of aaaalavandua vera using the “ open culture system” , Scientia Hort. 2000(86):81-88
14. Woltering,E.J. Beneficial effects of carbon dioxide on development of gerbera and rose plantlets grown in vitro,Sci.Hort.1990,44:341-345
15. Zobayed, S. M. A. ; Afreen, F., Kubota, C. et al Water control and Survial of Ipo moea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilation , Annals of Botany ,2000,86 : 603-610
16. Seko,Y. , Nishimura,M. Effect of CO2 and light on survial and growth of rice regenants grown in vitro on sugar-free medium,Plant cell,Tissue and Organ Culture 1996,46:257-264