Abstract: In order to lower the production cost, and make the fertilizer been convenient for the manufacturer to obtain in brief and easily, experiment was done to study the effect of different quantity of dry swine waste, NH4H2PO4 and composite fertilizer on the growth and development of cucumber in soilless culture. The results showed that the two more fertilizer treatments of dry swine waste 166.7g + NH4H2PO4 16.7g + composite fertilizer 16.7g per plant and dry swine waste 100g + NH4H2PO4 10g + composite fertilizer 10g per plant had the higher plant growth, chlorophyll content, carotenoidse content and photosynthesis rate. Except the nitrate content is higher than other treatments, the fruit quality such as soluble protein, total soluble sugar and Vc content is better than other treatments. The output of treatment dry swine waste 100g + NH4H2PO4 10g + composite fertilizer 10g per plant was not only higher than nutrient solution treatment, but also significant higher than other treatments. As a result, in eco-organic soilless culture system, the proper use of chemical fertilizer can improve plant growth and output of cucumber.