Cloning and Sequencing Analysis of ORF2 Gene of Porcine Circovirus 1

Abstract

Abstract: The ORF2 genome was amplified from PK-15 cells and tissues of piglets suffered from PMWS respectively using the primers designed according to the OFR2 sequence published in Genbank. Then the resultant PCR products were cloned into pMD18-T vector. The nucleotide sequence data of PCV1-ORF2 gene was analyzed by comparing with that of other PCV1-ORF2 and PCV2-ORF2 gene sequences. The results indicated that the nucleotide homology of ORF2 gene cloned from PK-15 cells and pig tissues was 99.4%, the homology of all selected PCV1-ORF2 genes was 97.3%~99.9%, and the homology of PCV1-ORF2 and PCV2-ORF2 was 67.9%~68.4%. Meanwhile, several conserved founctional domains were discovered in PCV1-ORF2 gene, and the forecast of antigenicity indicated the protein translated by PCV1-ORF2 gene having well immunogenicity.

CLC Number:

S852.659.2

Jin Yufen, Hao Guizeng, He Hongxuan. Cloning and Sequencing Analysis of ORF2 Gene of Porcine Circovirus 1[J]. Chinese Agricultural Science Bulletin, 2007, 23(9): 3-3.

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