Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (9): 3-3.

Special Issue: 畜牧兽医

• 畜牧兽医科学 • Previous Articles     Next Articles

Development of PCR for Diagnosing Isospora Suis

Liu Guanghui, Ning Changshen, Zhang Longxian,Cheng Junzhen, Jian Fuchun   

  • Online:2007-09-05 Published:2007-09-05

Abstract: One pair of primer(P1:5'-tcctgcgagtactcatatgc-3';P2:5'-gttcagctacgcataccttg-3') was designed by using Oligo 6.57,Primer Premier 5.0 according to the sequence published by the GenBank. 18SrRNA gene of I.suis which isolated from different area in Henan Province was amplifed succesfully for the first time by the primer. The result was analysis by Paup and Phylip revealed that the homology of I.suis isolated from different area in Henan Province was 100%,and there was no variation consisted in Henan Isospora suis

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