Abstract: 【OBJECTIVE】DNA labeling technology is important for research of molecular and genetic engineering, So it is important to develop a high-efficiency, rapid, low-cost, safe, simple and convenient method for labeling DNA; 【METHOD】Based on PCR principle, DNA was labeled using Taq DNA Polymerase and the result was detected by electrophoresis and southern-blot.【RESULTS】The electrophoresis results indicated the product labeled was lag than control DNA, which improved that the label succeed; The bright and clear strip indicated the labeling efficiency was high; Cross strip in southern-blot was clear, which proved the product labeled by the method can be used in molecular hybridization. 【CONCLUSION】PCR labeling using Taq DNA polymerase is a low-cost, high-efficiency and rapid DNA labeling method