Abstract: In order to gain the follicular oocytes which was high quality, and improve the frequency of in vitro production of the goat’s embryos, the dissection method and the aspiration method were applied to collect the follicular oocytes of the goats, and the available oocytes were used to be in vitro maturation. The results showed that the available oocytes obtained from the dissection method was more than that from the aspiration method, and number of the oocytes used to be in vitro maturation was obviously increased. The mature rate of the oocytes was closely related with the follicular diameter, and the mature rate of the oocytes on the big follicle and on the medium follicle was obviously higher(P<0.05) than that on the small follicle. The added proportion of FCS was useful between 10% ~ 15% in the mature culture solution of the follicular oocytes. The increase of FCS’s concentration couldn’t improve the mature rate of the oocytes in the range.