Abstract: Tissue culture of shoot segments with side buds of Aglaonema commutatum cv. ‘Golden Jewelry’ were studied in present paper. Treatment with different concentration of 6-BA was attempted for adventitious shoot induction. Lower concentration（1.5 mg/L） of BA was suitable. MS+BA2.5 mg/L+NAA0.05~0.1mg/L was better for buds proliferation with the proliferation coefficient 4.1every month. Seedling transferred in 1/2MS medium with 0.2~0.5mg/L NAA could root normally.