Abstract: In order to isolate high quality DNA from Actinidia leaves enriching polysaccharides and polyphenols and preserved from field samples, the effects of DNA extraction were studied with four different methods including CTAB regular method, CTAB subarea method, SDS regular method, SDS subarea method and three different conditions of storing sample (frozen at -20℃ and -70℃, dried with silica gel). Moreover, the CTAB subarea method was used for the first time for genomic DNA isolation from Actinidia .The results showed that the CTAB subarea method and dried with silica gel was optimal, the value of OD260/OD280 was over 1.8,the DNAs were completely digested by restriction endonuclease and the extracted DNA by this method was appeared as a clear band when electrophoresed in 1% agrarose gel, and produced clear polymorphic patterns amplified by PCR .