Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (12): 77-77.

Special Issue: 生物技术

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cDNA-AFLP Technique and Its Application in Cloning of Differentially Expressed Genes

Yu Hong, Ding Guohua,   

  • Online:2007-12-05 Published:2007-12-05

Abstract: cDNA-AFLP technology, which is used mainly for studying genetic difference, and developed on AFLP technological foundation, and had the reliable advantage and high-efficient, is favored by researcher and widely used in the research of all kinds of biological gene expression including plant. This article elaborated briefly the cDNA-AFLP technology principle, discussed emphatically the issue and the technical key which in the operating process possibly appears, and have carried on the comparison on this technology with other difference expression technology, explained it expresses in the gene in the clone difference the superiority, has enumerated briefly some examples in disease resistant breeding application.

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