Cloning and Expression Analysis of Boron influxTransporter NIP5;1 from Nicotiana tabacum

doi:10.11924/j.issn.1000-6850.casb16030094

Abstract

Abstract: To understand the mechanism of boron transportation in tobacco, the boron influx transporter gene was cloned in Nicotiana tabaccum, and its expression was analyzed. The open reading frame (ORF) of boron influx transporter was predicted by biology informatics methods, and cloned by PCR technique, verified by sequencing. Its structure was also predicted by biology informatics methods. Semi-quantitative RT-PCR was used to monitor its expression levels in different tissues of tobacco with different treatments. Results showed that the complete ORF sequence of tobacco influx boron transporter, NtNIP5;1 was cloned. Its ORF contains 894 bp, encodes 297 amino acids and includes MIP protein signature sequence HLNPSLTIA. GenBank accession number is KF611892. Its protein consists of α-helix mainly in secondary structure, and was composed of five transmembrane domains. Results of RT-PCR showed that NtNIP5;1 were expressed in roots and leaves, and showed higher levels in leaves. Its expression was inhibited by 6-BA, SA and ABA.

CLC Number:

Q781

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