Abstract: With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA、IBA 、2,4-D. The result indicated that (1) The effect sequence of auxin was 2,4-D > NAA >IBA from the callus induction. (2) Callus were induced on medium of different hormone combinations，and MS+BA1.0~2.0 mg/L+NAA 0.5~1.5 mg/L、 MS+BA1.5~2.0 mg/L+ IBA1.0~1.5mg/L、 MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5mg/L etc. was the best one for callus induction，and the rate of induction was respectively 74.3%、65.3%、81.1%. So through scientific preparation，callus induction of young stem can be induced effectively，on MS medium with different hormone combinations.