Abstract: The experiment has solved basically some key problems of the technology of the clonal propagation and the factory culture seedling of Pseudostellaria heterophylla. Several new media which suited different growth stage have got through the experiment. The medium used for shoot-tip culture is MS+NAA 0.5mg/kg+BA 0.5mg/kg. The planting formation ratio is 86.7%. The media for subculture are MS+NAA0.5mg/kg+BA0.5mg/kg and MS+NAA1.0mg/kg+BA0.5 mg/kg. The medium of growing root is 1/2MS+IAA1.0mg/kg, the ratio of rooting is 76.7%. The concentration of sucrose of every medium is 3%. The living ratio of the transplantation of shoot-tip cultured plantings is 85.2%. The living ratio of the field plantings is 100%. A simple, quick and practical production process of factory culture seedling of Pseudostellaria heterophylla has been successfully established.