Abstract: Abstract: 6 positive clones of transcription elongation factor B (S III) (Tceb2) gene were screened from the cDNA library of alpaca skin with Southern Blotting. After being sequenced and treated, the sizes of the inserted fragments was 472bp with the complete open reading frame. It was encoded 119AA with MW13.2KDa. Analyzing the characteristics, the structure of the sequence and the similarity, we found that the sequenced was the predicted full-length cDNA and it had 100% similarity with that of mouse and rat. So it was named as alpaca Tceb2 (GenBank DQ646397).