Abstract: Endogenetic Bacillus coagulans R14 and B. licheniforms R21 isolated from Dendrobium leaves were used to control leaf spot of Dendrobium caused by Fusarium moniliforme B10b. The growth of B10b could be inhibited by R14 or R21 with the inhibition zone 4.94 mm or 6.33 mm respectively on petri dish, and the mycelium of B10b was found to be killed by these two strains. The germination of B10b spore could be greatly inhibited by R14 or R21 fermenting liquor, the EC₅₀ were 538.270μl/ml or 498.884μl/ml. Greenhouse test showed that leaf spot of Dendrobium caused by B10b could be effectively controlled by R14 and R21, the efficacy was up to 70.13% and 67.53% respectively.