Abstract: The effect of different explants and different exogenous hormones on callus induction of chestnut short catkin mutant was studied regarding MS as basic medium. The results showed that the optimal inducing medium was MS + 1.0 mg/L 6-BA + 0.2 mg/L NAA, and callus inducing rate using young stem as explants was higher which was 65.0% compared with young leaves. Callus propagation was subcultured on available medium of MS + 0.5～1.0mg/L 6-BA + 0.2mg/L NAA.