Abstract: The mature embryos of 5 old years?cultivars of 慞remier?honey peach were used as the materials in this paper. The effects of the inoculation method, light treatment and different mediums on the callus induction were studied. The results showed that the friable and well callus were obtained by the cotyledonal embryos without spermoderm on the MS with 2.0 mg/L 2,4-D under the dark. The comparisons of the effects of the light treatment, callus type, different mediums and subculturing method on the growth of the honey peach callus were investigated in the process of their subculture and maintenance. The conclusions were summarized as follows, the TN2 and TN3 of the honey peach callus could be maintained for a long time and kept exuberant growth when they were alternately cultured on the MS medium containing with 1.0 mg/L 2,4-D and the other MS medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L KT and 100 mg昄-1 inositol.