Abstract: Abstract: The extraction and isolation conditions of Agaricus.Blazei. Murrill （ABM） polyphenol by supercritical CO2 process were studied in this experiment. Influences of indexs which were about extraction temperature, extraction pressure, extraction time were investigated，and suitable conditions were determined based on the single-factor and orthogonal experimental tests. They were as follows: 60% ethanol, extraction temperature 75℃, extraction pressure 40 MPa, CO2 Current capacity 20L/h, the amount of entrainer 120mL. And then compared with static state extracted and dynamic extracted, static state extracted 1 hour, and then dynamic extracted 1 hour was best, extraction rate could reach to 2.431%.