Abstract: In the present study, both Salicylic (SA) acid and nitric oxide (NO) were proven as signaling molecules in regulating a serial of physiological processes in plants. Using pharmacological and biochemical approaches, we evaluated the protective effect of exogenous salicylic acid against salt-induced oxidative damage in the roots of wheat seedling. Plants exposed to salt (150mmol/L NaCl) exhibited the significant increase of lipid peroxidation as well as the inhibition of root growth. However, SA (100μmol/L) supplementation to NaCl (150mmol/L) treated plants effectively not only reduced the lipid peroxidation and partially increased the root growth, but also activated anti-oxidant enzyme, including superoxide dismutase (SOD), activities.guaiocol peroxidase (POD) and ascorbate peroxidase (APX), which was also mimicked by the application of the nitric oxide (NO) donor sodium nitroprusside (SNP). Further analyses showed that the above ameliorating effects of SA were specific and related to NO, because the specific scavenger of NO 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (cPTIO) differentially blocked the above effects. Additionally, SA was able to mimic the effect of SNP by strongly increasing NO release in the roots after daily treatment. Taken together, above results suggest that SA might confer an increased tolerance to salinity stress by enhancing antioxidant defense in wheat seedling roots, which were partially mediated by NO signal.