Abstract: To learn the vigor and physiological characteristics of maize in ageing course ,Seeds of two maize inbred T6 and inbred NT6621 were used in their artificial ageing course .The result showed : the germination index and dehydrogenase activity of these materials reduced gradually after been treated with prolong of aging disposal under the no air conditions of high temperature(45±1℃) and high humidity relative humidity 100 percent),but the MDA content increased as the seeds aging treating time extension. eletrophretic of esterase experiment，The esterase activity of NT6621 and T6 were reduced after aging 24 hours .So the esterase activity was reduced with the aging.