Abstract: Abstract: The activities of phospholipase D in response to cold stress had been studied using artificial climate chamber in the seedling stage of winter wheat. The experimental data showed that the activities of PLD had been highly expressed in leaves at germinating and seedling stage. In response to cold stress, PLD was transiently induced regardless of wheat cultivars, and the activity change pattern of cold-sensitive winter wheat cultivar was different with cold-resist cultivars. Taken together, our data suggested a role of PLD in cold stress in plants.