Abstract: Elite germplasm resource is the base of wheat breeding. The protein quality of 261 Chinese mini-core collection germplasms and 54 application-core collection germplasms were identified in 2006 and 2007 in Yangzhou. 19 germplasms with low protein content and 12 germplasms with low SDS-sedimentation value were screened out, 4 of them could be determined as high quality weak gluten germplasms, and be used in weak gluten wheat breeding.