Abstract: 【OBJECTIVE】The purpose of this paper was analyze the characters of the wheat variety which had experienced the space. 【METHOD】Yan2070, Yannong24, Yannong19, Yannong21, which had sufficient purity and quantity, were used and experienced the space light of “Shi Jian 8”. 【RESULTS】The results showed that the characters of wheat seedling stage appeared the distinct changes. SP1 plants had high germination rate, low emergence rate and seedling survival rate, and occurred albino seedlings. But SP1 plants didn’t show distinct changes in tillering ability and frost resistance. This indicated that space flight may reduce the ability to resist environment condition of wheat at seedling stage. The variation rates of four wheat lines were 2%~3%. The characters of SP1 variation plants at maturity stage also appeared big changes. SP1 variation plants had short growing period, high seed setting rate, loose plant-type and clavated spike-type. There were not consistent change in plant height, the number of spike grain and the weight of 1000 kernel among four lines. 【CONCLUSION】Some variations mutated by space flight could appeared at the present generation. The variation of space flight may advantageous or disadvantageous, and may stochastic or be related with the line genetic characteristics.