Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (12): 44-49.
Special Issue: 生物技术
• 农业生物技术科学 • Previous Articles Next Articles
Wang Ying, Qiu Haiyan, Gao Heqiong, Huang Dongyi, Zhuang Nansheng
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Abstract: The sugarcane tender leaves were taken as experiment materials, through made the improvement and the experiment to predecessors CTAB methods to reform a better extraction method of sugarcane genomic DNA. The final improved CTAB method (method Ⅲ) had the characteristics of costing low, operation simple, time-saving and so on. Ultraviolet absorbance spectrum analysis, agarose gel electrophoresis and PCR technique showed that DNA samples obtained by method Ⅲ had a good integrity and high purity, could be used in each kind of molecular markers directly.
Wang Ying, Qiu Haiyan, Gao Heqiong, Huang Dongyi, Zhuang Nansheng. Study on Extraction Methods of Sugarcane Genomic DNA[J]. Chinese Agricultural Science Bulletin, 2008, 24(12): 44-49.
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https://www.casb.org.cn/EN/Y2008/V24/I12/44