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Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (12): 50-53.

Special Issue: 生物技术

• 农业生物技术科学 • Previous Articles     Next Articles

High-level Expression Ramie Caffeoyl-CoA 3-O-methyltransferase Gene in E.coli

Chen Jianrong, Guo Qingquan, Zhang Xuewen, Chen Jinjun   

  • Received:2008-08-15 Revised:2008-10-07 Online:2008-12-05 Published:2008-12-05

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Abstract: Ramie caffeoyl-CoA 3- O-methyltransferase was expressed by pQE High-level Expression system in E.coli. The molecular weight of Ramie Fusion caffeoyl-CoA3- O-methyltransferase is 29.4 kD. The structure of the enzyme has been constructed by SwissModel server. Multiplex structure of the enzyme ,Sinapoyl CoA , SAH and Ca2+ has been constructed by Docking module in software InsightII,and has been optimized by Discover module. It can be presume that Ramie caffeoyl-CoA 3- O-methyltransferase has catalyse function of O-methyltransferase, must been related to lignin monomer precursor methyltransfer.