The Effect on Chimeric Embryo Construction from Fibroblasts and Early  Embryos about Mouse Embryoid Body

Abstract

Abstract: Chimeric embryos from mouse embryos and human fibroblasts were constructed. The fibroblasts from adult foreskin were isolated, cultured and transfected EGFP with lentivirus vectors, and then chimeric embryos were constructed with mouse embryos and fibroblasts. The results showed that human fibroblasts efficiently expressed EGFP after transfected with lentivirus vectors. 38.08 % chimeric embryos were constructed successfully with mouse embryos and EGFP fibroblasts which were from embryoid bodies of mouse embryonic stem cells and EGFP fibroblasts. EGFP fibroblasts were taken part in different ingredient of chimeric embryos. Under the circumstance of embryonic stem cell isolation and culture, EGFP fibroblasts in 1.74 % chimeric embryos became the member of inner cell masses on feeder cells. Human fibroblasts can become chimeric embryos with mouse embryos by the microenvironment of mouse embryoid bodies.

CLC Number:

Q813.5

Cao Hongguo, Li Yunsheng, Qu Xiuxia, Deng Hongkui. The Effect on Chimeric Embryo Construction from Fibroblasts and Early
Embryos about Mouse Embryoid Body[J]. Chinese Agricultural Science Bulletin, 2008, 24(9): 25-30.

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The Effect on Chimeric Embryo Construction from Fibroblasts and Early
Embryos about Mouse Embryoid Body

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