Abstract: In this experiment the ramification from tea adult stem nodes was used as materials for the preliminary study on subculturing multiplication and rooting formation in Camellia sinensis cv Tieguanyin. The obtained results showed that the best medium for subculturing multiplication of tea adult stem nodes was 1/2MS＋1.0 mg/L6-BA＋0.2mg/LIAA＋0.2mg/LGA3 supplemented with 8g/L agarose and 30g/L sucrose, pH5.6. The average multiplication rate was 7.57 and the highest could come up to 13. The optimal medium for rooting formation of aseptic lines from adult stem nodes was 1/2MS＋3mg/LIBA＋30g/L sucrose＋7g/L agarose; further more the rooting rate improved with prolonging subculturing time, while the days of rooting formation shortened.